Uncaria gambir Roxb., salah satu tanaman asli Indonesia yang mengandung katekin dengan kadar yang tinggi. The purity of catechin isolate obtained was 99.80%± 0.132. Catechin purification was monitored using Thin Layer Chromatography (TLC) method with eluent chloroform:ethyl acetate:formic acid (5:4:1, then identified using High Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance (NMR) Spectroscopy, and Liquid Cromatography- Mass Spectroscopy (LC-MS). Pure catechin isolate was obtained using a vacuum liquid chromatograpy method with a series of solvent hexane and ethyl acetate gradients. Isolation of catechin from gambir extract was done by percolation method using ethyl acetate solvents.

Gambier extract is characterized according to the standard method stated in Farmakope Herbal Indonesia. Gambir extract used for catechin isolation was a quality 1 gambir extract obtained from Padang, West Sumatra. extract was carried out, which will then be the starting material in the derivatization of catechin. In this study, isolation and purification of catechin isolate from Uncaria gambir Roxb. Derivatization process needs pure catechin isolate as a starting material in order to obtain maximum result so that the isolation process is one of the important stages. Derivatization of catechin can be developed to produce drug compounds that are effective as antiviral agents for HIV. Catechins are very potential to be used for medicinal raw materials because theirs effects are proven to be antibacterial, antiviral, and antidyslipidemic. Uncaria gambir Roxb., one of native plants of Indonesia contains high levels of catechins.

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